Tuberin antibody

Catalog No. ABIN7674572

This Rabbit Polyclonal antibody specifically detects Tuberin in ELISA, WB, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Tuberin antibody (ABIN7674572)

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tuberin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Tuberin Antibody

Purpose: TSC2-Specific antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: tuberous sclerosis 2

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IHC: 1:20-1:200,IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for Tuberin

Target: Tuberin (TSC2) (Tuberous Sclerosis 2 (TSC2))

Alternative Name: TSC2

Background:

Synonyms: Tuberin|Tuberous sclerosis 2 protein|TSC2|TSC4

Background: TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein(GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1(hamartin) gene cause tuberous sclerosis complex(TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. The antibody is specific to TSC2.

Molecular Weight: 200 kDa

Gene ID: 7249

UniProt: P49815

Pathways: RTK Signaling, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus